Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved Microarchitecture

The application of decellularized scaffolds for artificial tissue reconstruction has been an approach with great therapeutic potential in regenerative medicine. Recently, biomimetic ovarian was proposed to reestablish endocrine functions. Despite many decellularization methods proposed, there is no established protocol whole ovaries by detergent perfusion that able preserve macro and microstructure higher efficiency. This generated biomaterial may have the be applied other purposes beyond reproduction translated areas engineering field. Therefore, this study aimed establish standardize a porcine ovaries’ based on ultrasonication obtain functional whole-ovary scaffolds. For that, (n = 5) were perfused detergents (0.5% SDS 1% Triton X-100) submitted bath produce acellular efficiency evaluated DAPI staining total genomic DNA quantification. ECM morphological evaluation performed histological, immunohistochemistry, ultrastructural analyses. physico-chemical composition using FTIR Raman spectroscopy. A cytocompatibility cell adhesion assay murine fibroblasts performed. Results showed method remove cellular components efficiently. There significant component loss relation native tissue, cytocompatible allowed attachment. In conclusion, produced whole-ovaries preserved engineering.